Isolation of Enteropathogenic Escherichia coli from Children with Diarrhoea Attending the National Hospital in Abuja, Nigeria.

[摘要] Background::Diarrhoealdiseasesremainoneoftheleadingcausesofchildhoodmorbidityandmortalityinmostdevelopingcountries,withenteropathogenicEscherichiacoli(EPEC)beingoneofthemostimportantaetiologicagentsofinfantilediarrhoeainmanyofthesecountries.Objective::TodeterminetheprevalenceofEPECinchildren(0-24months)withdiarrhoea.Methods::OnehundredstoolsamplesfromchildrenwithdiarrhoeaattendingtheNationalHospitalinAbuja,NigeriawereanalysedforthepresenceofEPECusingstandardbacteriologicalmethods.Isolateswerealsosubjectedtoantimicrobialtestingusingthediscdiffusionmethod.Results::Fifteen(15.00%)sampleswerepositiveforEPEC.Thehighestnumberofisolateswasrecoveredfromthe6-12monthsagegroupwith10(20.41%)intotal.Noisolateswererecoveredfromthe0-5monthsagegrouporfromexclusivelybreastfedchildren,however,nine(19.15%)childrenreceivingmixedfeedingandsix(33.33%)childrenwhowerenotbeingbreast-fedtestedpositiveforEPEC,andthedifferencewasfoundtobestatisticallysignificant(P#60;0.05).Thehighestnumberofisolates,eight(53.33%),belongedtothoseinthepoly1sero-groupwithserotypesO1,O26,O86a,O111,O119,O127andO128.Fourteen(93.33%)oftheisolatesweresensitivetonorfloxacin,13(86.67%)eachtoofloxacinandcolistin,11(73.33%)tonalidixicacid,10(66.67%)tocefuroxime,two(13.33%)tocotrimoxazole,andone(6.67%)totetracycline.Noneoftheisolateswerefoundtobesensitivetoampicillinorchloramphenicol.Conclusion::ThisstudyhasshownthatmultidrugresistantEPECisassociatedwithinfantilediarrhoeainAbuja,Nigeria.Toaddressthisissue,antibiotictherapyshouldtakeintoconsiderationthesusceptibilitypatternofthepathogen.Inaddition,theincidenceofEPECinchildrencanbetracedprimarilytofaultyweaningpracticesand/orpoorpersonalhygiene.

[学科分类] 基础医学

[关键词] Enteropathogenic Escherichia coli (EPEC);diarrhoea;children;serotype;Abuja;Nigeria