Characterization Of Two Soluble (52 / 42 Kda) Glycoproteins Whose Pattern Is Up-Regulated During Yeast-To-Filament Growth Transition Of C. Albicans.

[摘要] Inthisstudy,wesoughttoinvestigatethepatternofcellularglycoproteinsduringyeast-to-filamentgrowthtransitioninCandidaalbicans,invitro.BymeanofglycoproteinextractionwithConcanavalinA-Sepharose,andWesternBlotanalysiswithperoxidase-labeledlectins(concanavalinA,wheatgermagglutinin),wehavecharacterizedtwoglycoproteins(52kDaand42kDa)whosepatternisincreasedinthefilamentousformofC.albicans.AnalysisofsubcellularfractionsofC.albicansshowedthatthe52/42kDaglycoproteinsarelocatedinthesolublefraction.invitrotreatmentofconcanavalinA-Sepharoseextracted52/42kDaglycoproteinswiththepeptideN-linkedglycosidaseFshowedthatthe52kDaproteinishighlyN-glycosylatedandmannose0-glycosylated,whereasthe42kDaisN-glycosylated.Regulationofsynthesisand/orglycosylationofthe52/42kDaglycoproteinscouldbeassociatedwithyeast-to-filamentgrowthtransitionofC.albicans.

[学科分类] 基础医学

[关键词] C.albicans;filamentous growth;protein glycosylation;concanavalin A;wheat germ agglutinin.