Molecular Biological Studies of the Induction of Flowering

[摘要] Potential changes in gene expression which occur in the leaves during photoperiodic floral induction were investigated in a variety of plant species including Nicotiana plumbaginifolia (day neutral plant), Amaranthus caudatus (short day plant), Silene coeli-rosa (long day plant), and Anagallis arvensis (long day plant) which exhibit a range of photoperiodic responses. The approach taken was to employ techniques which would demonstrate differential gene expression at the level of mRNA or protein including in vitro translation of leaf mRNA and analysis of products using one- or two-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)1analysis of leaf total protein using one- or two-dimensional SDS-PAGE, differential immunoadsorbtion techniques for the identification of 'induced' specific proteins, and differential screening of an 'induced' cDNA library.

[发布机构] University:University of Glasgow

[关键词] Botany, Plant sciences