D(-)-Mandelate Dehydrogenase of the Yeast Rhodotorula graminis

[摘要] 1. The yeast Rhodotorula graminis strain KGX 39 can grow on D(--)- or L(+)-mandelate as a sole source of carbon and energy. The first step in the dissimilation of mandelate involves stereospecific oxidation to phenylgiyoxylate, and this is then degraded via benzaldehyde to benzoate. Preliminary evidence had indicated the presence of a dye-linked L(+)-mandelate dehydrogenase and an NAD+-dependent D(--)-mandelate dehydrogenase in this organism. This thesis is concerned with the purification and characterization of the NAD+-dependent D(--)-mandelate dehydrogenase.

[发布机构] University:University of Glasgow

[关键词] Biochemistry