Growth of human breast cells in primary culture

[摘要] Human breast tumours were enzymatically disaggregated with collagenase and established in primary culture on a variety of feeder layers of mitomycin-C treated mouse embryo fibroblasts. The NIH-3T3 and STO cell lines proved the most successful in promoting the growth of primary breast cultures (successful cultures from approximately 80% of breast tumours).

[发布机构] University:University of Glasgow

[关键词] Cellular biology, Biochemistry