Is a Schiff base involved in the mechanism of the Δ4-3-oxo steroid 5α- or 5β-reductases from mammalian liver?

[摘要]

The saturation of the delta4-double bond of delta4-3-oxo steroids by the mammalian 5alpha- or 5beta-reductases is by an enzyme mechanism that does not involve a Schiff-base intermediate.