Organization and In Vitro Expression of Human tRNAGlu Genes

[摘要] Prior to the start of this work several recombinants containing tRNA genes were selected from a bank of 15-20 kb fragments of a human foetal DNA, cloned in l Charon 4A,by screening with a mixed placentae tRNA[32P]pCp probe (Goddard et al. , 1983). Two of these recombinants, lht137 and lht190, generated restriction fragment profiles which were very similar but not identical. Two common 2.4 kb Hindlll fragments which hybridized the probe used, were subcloned from lht137 and lht190 into the vector pAT153 to generate recombinants pLB4 and pTC51 respectively. The characterized recombinant pLB4 was found to contain a single tRNA Glu gene, notable for its high transcriptional activity and the potential of its 5'-flanking sequence for forming a tRNA-like structure (Goddard et al., 1983).

[发布机构] University:University of Glasgow

[关键词] Biochemistry, Genetics