Short-term radiorespirometry of cell suspensions

[摘要]

1. An apparatus and method are described with which the oxidation of labelled substrates to¹⁴CO₂ by cell suspensions may be examined. 2. The use of high-specific-radioactivity substrates at low concentration together with frequent quantitative collection of CO₂ permit a more detailed analysis of the appearance of various substrate carbon atoms as CO₂ than is possible by existing techniques. 3. Typical experiments with various cell types are reported, in which pathways of glucose oxidation are examined.