Studies of the Initial Steps of Lysine Biosynthesis in E. coli

[摘要] 1. A pUC9 derived plasmid, pDA2, containing the gene encoding for DHDPA synthase was obtained from Dr. P. Stragier in Paris. The E. coli strain MV1190 (RecA-) was transformed by pDA2 to produce an overexpressing strain for DHDPA synthase. The specific activity of this crude extract showed 200-fold more activity than that from the wild-type.

[发布机构] University:University of Glasgow

[关键词] Biochemistry