Studies on the pathogenesis of liver necrosis by α-amanitin. Effect of α-amanitin on ribonucleic acid synthesis and on ribonucleic acid polymerase in mouse liver nuclei

[摘要]

1. Injection of α-amanitin to mice causes a decreased incorporation of [6−¹⁴C]-orotic acid into liver RNA in vivo. 2. The activity of RNA polymerase activated by Mn²⁺ and ammonium sulphate is greatly impaired in liver nuclei isolated from mice poisoned with α-amanitin, and is inhibited by the addition of the same toxin in vitro. 3. The activity of the Mg²⁺-activated RNA polymerase is only slightly affected by α-amanitin either administered to mice or added in vitro.