The Structure and Expression of Genes Encoding the Small Subunit of Ribulose 1, 5-Bisphosphate Carboxylase/Oxygenase in Phaseolus vulgaris L

[摘要] The multigene family encoding the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase [EC 4.1.1.39] in Phaseolus vulgaris L. contains,at least three members. cDNA clones representing 3 members of the family (rbcS genes) have been characterised in terms of structure and expression. These rbcS genes show very high nucleotide sequence homologies in the coding regions for both the transit peptide and the mature polypeptide. The nucleotide sequences for the 5' - and 3' -untranslated regions of these genes which were present in the cDNA clones showed far more divergence. One of the rbcS cDNA clones contained two regions of intronic sequences, the first lying between the codons for the 2nd and 3rd amino acids of the mature polypeptide and the second lying between the codons for the 47th and 4 8th amino acids of the mature polypeptide.

[发布机构] University:University of Glasgow

[关键词] Biochemistry