NEW MUTATIONAL VARIANTS OF NEUROSPORA NADP-SPECIFIC GLUTAMATE DEHYDROGENASE

[摘要] The am locus of Neurospora codes for NADP-dependent glutamate dehydrogenase (GDH). Four new am mutants that produced mutationally altered GDH have been characterized. Mutant am 119 is a CRM-negative, complementing mutant that maps between am 2 and am 1. The other three mutants are CRM formers that produce varieties of GDH that can be activated by glutamate or succinate. The GDH of am 130 and am l31 is similar in terms of activation properties to that of am 3. The GDH of am l22, requires very high concentrations of dicarboxylate for activity. The mutation in am l30 maps between am l4 and am 2 and resulted in a replacement at residue 75 of the GDH (pro→ser). The mutation in am l22 maps near am ll and apparently resulted in the replacement of the tryptophan residue at position 389 with an unknown amino acid. The mutation in am l31 maps between am 2 and am 1.

[学科分类] 医学（综合）