A METHOD FOR COMPLEMENTATION ANALYSIS OF NUCLEAR AND CHLOROPLAST MUTANTS OF PHOTOSYNTHESIS IN CHLAMYDOMONAS

[摘要] The photosynthetic properties of young zygotes of Chlamydomonas reinhardi were analyzed. In heterozygotes for two nuclear or two chloroplast mutations affecting photosynthesis, recovery of photosynthetic activity was observed that is most likely the result of intergenic complementation.—We observed that chloramphenicol inhibited the recovery of activity in double heterozygotes for mutants lacking at least one thylakoid polypeptide of chloroplast origin, while it had no effect on wild-type homozygotes. This indicates that the recovery of activity in double heterozygotes could result from the repair of existing thylakoid membranes by de nouo synthesis of the missing polypeptides.

[学科分类] 医学（综合）