ASYMMETRIC EFFECTS OF DELETIONS AND SUBSTITUTIONS ON HIGH NEGATIVE INTERFERENCE IN COLIPHAGE LAMBDA

[摘要] Experiments have been performed to help clarify the role of nonhomologies in phage λ recombination. Three-factor crosses were carried out, and the frequencies of single and double recombinants in the two adjoining intervals were compared when the central marker was either a double point mutation ( v1v3 ) or deletion ( rex-cI deletion) or nonhomologous substitution ( imm434 ). In all cases the lefthand marker was a bio substitution (Fec- phenotype, which does not permit plating on recA -), and the righthand marker was an amber mutation in gene O . Experiments were performed in all four possible arrangements of the central and rightward markers, while selecting for the Fec+ phenotype on the recA - host. As anticipated, high negative interference (HNI) was observed with point mutations, but when the central marker was a substitution nonhomology, HNI was reduced about tenfold. Surprisingly, when the central marker was a simple deletion, a dramatic asymmetry in results was observed, with HNI being exhibited only when the central deletion marker was acquired by the double recombinant. These results indicate that under normal conditions ( red +, gam +, rec +) and with noninhibited DNA replication, recombination in coliphage λ entails a highly asymmetric step that could be at the level of strand transfer or mismatch repair.

[学科分类] 医学（综合）