ISOLATION AND GENETIC ANALYSIS OF CAULOBACTER MUTANTS DEFECTIVE IN CELL SHAPE AND MEMBRANE LIPID SYNTHESIS

[摘要] In this paper we report the isolation, characterization and genetic analysis of several C. crescentus mutants altered in membrane lipid synthesis. One of these, a fatty acid bradytroph, AE6002, was shown to be due to a mutation in the fatA gene. In addition to the presence of the fatA506 mutation, this strain was found to contain two other mutations, one of which caused the production of a water-soluble brown-orange pigment ( pigA ) and another which caused formation of helical cells ( hclA ). Expression of the latter two phenotypes required complex media and both were repressed by glucose. However, the lesions were mapped to loci that are separated by a substantial distance. The hclA and the fatA genes mapped close together, possibly implying that comutation had occurred in AE6002. Data are presented that allow the unambiguous identification of a second Fat gene ( fatB ) in C. crescentus . The map position of another mutation in membrane lipid biogenesis, the glycerol-3-PO4 auxotroph gpsA505 , was also determined. During this study the flaZ gene was fine-mapped and the positions of proC and rif changed from the previously reported location.

[学科分类] 医学（综合）