ACTIVITY OF CHI RECOMBINATIONAL HOTSPOTS IN SALMONELLA TYPHIMURIUM

[摘要] Chi sites have previously been shown to stimulate homologous recombination by the Escherichia coli RecBC pathway. To test the activity of Chi in another organism, bacteriophage λ crosses were carried out in Salmonella typhimurium strains bearing the E. coli λ receptor protein. Chi is active in these crosses in S. typhimurium , but is less active than in the same crosses carried out in E. coli . The lower Chi activity in S. typhimurium appears to be intrinsic to the S. typhimurium RecBC enzyme, since the Chi activity in E. coli-S. typhimurium hybrids depends on the species of origin of their RecBC enzyme. For these studies we constructed an F' factor and a pBR322-derived plasmid carrying the thyA + recC + recB + argA + region of the S. typhimurium chromosome.

[学科分类] 医学（综合）