GENETIC ANALYSIS OF LIVER NEURAMINIDASE ISOZYMES IN RATTUS NORVEGICUS: INDEPENDENT CONTROL OF NEU-1 AND NEU-2 PHENOTYPES

[摘要] Two recently identified isozymes of neuraminidase in rat liver were examined for transmission patterns and linkage relationships, and for variation among inbred strains. The isozymes, designated neuraminidase-1 (NEU-1) and neuraminidase-2 (NEU-2), exhibited no electrophoretic mobility variants among the 22 inbred strains examined, but did possess striking interstrain variation in activity phenotypes on electrophoretic gels. The results of a backcross analysis involving the KGH and ACP strains revealed that NEU-1 and NEU-2 phenotypes are independently controlled, each by a single autosomal locus with additively acting alleles. The two loci are unlinked to one another, but the gene controlling NEU-1 is tightly linked to RT1 , the rat major histocompatibility complex. This gene is almost certainly identical to Neu-1 , a gene identified previously through its effect on "total" activity levels of liver neuraminidase as determined by fluorometric assay of tissue homogenates. NEU-2 and the gene controlling its phenotype were not detected by the fluorometric technique. We designate the genes controlling the NEU-1 and NEU-2 phenotypes as Neu-1 and Neu-2 , respectively. Data from this and other studies place Neu-1 between Glo-1 and dw-3 . The location of Neu-2 is unknown.

[学科分类] 医学（综合）