MITOCHONDRIAL GENETICS. XII. AN OLIGOMYCIN-RESISTANT MUTANT LOCALIZED AT A NEW MITOCHONDRIAL LOCUS IN SACCHAROMYCES CEREVISIAE

[摘要] Three antibiotic-resistance mutations were isolated from strain FL496–2B: two are independent Mendelian genes, one conferring both oligomycin and venturicidin resistance ( oliR496 ) and the other conferring cycloheximide resistance ( cyhR496 ). The third is a mitochondrial mutation, OR9 , and confers a low level of oligomycin resistance to cells ( in vivo ) but not to the extracted mitochondrial ATPase ( in vitro ). This mutation is located on the mitochondrial DNA at a new locus [ OLI4 ] linked to [ OLI2 ] and independent from [ OLI1 ] and [ OLI3 ] and from the other mitochondrial loci. All three mutations ( OR9, oliR496, cyhR496 ) were found without any selection, in the same prototrophic haploid strain, which contained unknown resistances to antibiotics. Some physiological, genetical and biochemical properties of the mitochondrial mutation are described.

[学科分类] 医学（综合）