IDENTIFICATION OF A GENETIC ELEMENT THAT CONTROLS THE ORGAN-SPECIFIC EXPRESSION OF Adh1 IN MAIZE

[摘要] Allozyme balances serve as markers of quantitative behavior of electrophoretically distinguishable alleles. By the use of ADH Set I allozyme balances, it is demonstrated that all Adh1-S/Adh1-F individuals from more than 20 diverse S/F families exhibit a reciprocal correlation between Adh1 quantitative behavior in two maize organs: the scutellum and primary root. Within an electrophoretic mobility class, the Adh1 allele that is relatively underexpressed in the scutellum is relatively overexpressed in the primary root, and vice versa . Segregation tests prove that this "reciprocal effect" is the property of a cis -acting site that is closely linked to or within the Adh1 structural gene, and it is not affected by diverse genetic backgrounds. Immunological and [3H]-leucine incorporation experiments establish that Adh1 quantitative variants differ in ADH1·ADH1 synthetic rates in the anaerobic primary root. The reciprocal-effect phenomenon suggests that the cis -acting loci controlling Adh1 quantitative expression in each respective organ are at least in close proximity, or may share common DNA sequences. We discuss the possibility that the reciprocal-effect locus is a regulatory component of the Adh1 cistron .

[学科分类] 医学（综合）