Studies of a Rat Liver Ribonuclease Inhibitor Protein Gene

[摘要] The cytoplasmic ribonuclease inhibitor-ribonuclease system has been detected in a wide variety of tissues and species and is thought to play an essential role in the regulation of RNA metabolism and hence protein synthesis. This hypothesis is based on the observation that tissues with high levels of RNA and protein synthesis are associated with correspondingly high levels of free inhibitor and vice versa. However, the precise nature of this regulatory function is unknown. Therefore, the aim of this project was to isolate cDNA clones of ribonuclease inhibitor from a rat liver library in the expression vector, lgt11 using polyclonal antiserum, to characterise the resultant clones and to use the clones to determine the extent to which ribonuclease inhibitor activities are controlled at the level of gene expression.

[发布机构] University:University of Glasgow

[关键词] Biochemistry