RECIPIENT GENE DUPLICATION DURING GENERALIZED TRANSDUCTION

[摘要] An Hfrl3 ΔProA-lac deletion recipient, -ΔproA-lac-F-purE+-, has been utilized in a study of the origins of duplications formed during chromosome fragment integration. Among the Pro-Lac+ transductants, some have duplications spanning the F locus. These transductants are, or segregate, strains with F' episomes carrying genes of the duplication. Some of the duplications include purE+, a gene which is not coinherited with lac+ during bacteriophage P1- mediated transduction. Thus recipient genes have been duplicated during recombinant formation. Crossing-over models including replication steps provide a basis for explaining the duplication process.

[学科分类] 医学（综合）