REC-MEDIATED RECOMBINATIONAL HOT SPOT ACTIVITY IN BACTERIOPHAGE LAMBDA. I. HOT SPOT ACTIVITY ASSOCIATED WITH SPI-DELETIONS AND bio SUBSTITUTIONS

[摘要] In order to survey the distribution along the bacteriophage λ chromosome of Rec-mediated recombination events, crosses are performed using conditions which block essentially all DNA synthesis. One parent is density-labeled and carries a genetic marker in the left terminal λ gene (A) , while the other parent is unlabeled and carries a genetic marker in the right terminal λ gene (R) . Both parents are deleted for the λ recombination genes int and red , together with other recombination-associated genes, by virtue of either (1) a pure deletion or (2) a bio insertion-deletion. The distribution in a cesium density gradient of the resulting A+R+ recombinant phage reflects the chromosomal distribution of the recombination events which gave rise to those phage.

[学科分类] 医学（综合）