ISOLATION OF P22 SPECIALIZED TRANSDUCING PHAGE FOLLOWING F'-EPISOME FUSION

[摘要] A P22 specialized transducing phage has been constructed which carries the structural gene for aspartate transcarbamylase (ATCase). This gene ( pyrB ) was first brought close to the P22 attachment site by fusing an F' pyrB + episome to an F' prolac episome which carries a P22 prophage attachment site. A prophage was added to these fused F' episomes and the lysogen was UV-induced. The specialized transducing phage was isolated from the resulting lysate. The phage also carries argI , the structure gene for ornithine transcarbamylase.

[学科分类] 医学（综合）