Appearance of a Novel Type of Ganglioside (GD1α) in a Differentiation-Resistant Clone of Mouse Myeloid Leukemia Cells, M1-R1

[摘要] References(32)Cited-By(2)Glycolipid compositions of three mouse myeloid leukemia cell clones, two that are sensitive to differentiation inducers (M1-T22 and M 1-S1) and one that is differentiation-resistant (M1-R1), have been compared. The T22 and S1 clones contained glucosylceramide (GlcCer), lactosylceramide (LacCer) and gangliotriaosylceramide (Gg3Cer) as the major neutral glycolipids. The differentiation resistant clone, R1, was characterized by the appearance of globotriaosylceramide (Gb3Cer) and a decrease of Gg3Cer. There was a distinct difference in the ganglioside profile between the differentiation-inducible and-resistant clones: T22 and S1 cells contained no detectable amounts of ganglioside, whereas six different gangliosides were detected in the R1 clone. These gangliosides were isolated and identified as GM3, GM2, GM1a, GD1a, GMib, and a unique disialoganglioside, GD1a, having the following structure:Sialic acid α2 6GalNAcβ1-4Ga1β1-1Cer.Sialic acid α2-3Gal 3Based on these comparative studies, the relationship between the glycolipid composition and the differentiation potential of leukemia cells is discussed.

[学科分类] 分子生物学，细胞生物学和基因