Preparation and Culture of Spirogyra and Zygnema Protoplasts

[摘要] References(16)Cited-By(6)Protoplasts were enzymatically released from Spirogyra and Zygnema. The released protoplasts cultivated in liquid media regenerated a wall, grew in filamentous fashion and usually divided with an incomplete septum. When the media osmolarity was gradually reduced, the regenerated cells grew further. These regenerated cells appeared to be the same as the original cells.

[学科分类] 分子生物学，细胞生物学和基因