Studies on the Structure of Chromosomes I. The Uncoiling of Chromosomes Revealed by Treatment with Hypotonic Solution

[摘要] References(15)Cited-By(3)An attempt was made to reveal the chromosomal structure of Raji cells by light microscopy after treatment with several kinds of hypotonic saline solutions. Among various concentrations of sodium citrate, 0.4 % solution was found suitable for analysis of chromatid spiral structures. Similareffects of uncoiling on chromatids were obtained by solutions of 0.81 % potassium citrate, 0.3 % potassium acetate, 0.74 % guanidine chloride and 0.78% Veronal buffer, and 1/6 isotonic solution of sodium chloride (0.15 %) and potassium chloride (0.18 %). The mode of uncoiling, however, was somewhat different from solution to solution probably reflecting the action of electrolytes on cellular permeability.

[学科分类] 分子生物学，细胞生物学和基因