Reversal of ATP Inhibition of Phosphofructokinase by Nucleotides and Phosphate as a Possible Regulatory Mechanism of Sea Urchin Egg Glycolysis

[摘要] References(13)Cited-By(5)ABSTRAKT. Phosphofructokinase from eggs of the sea urchin Hemicentrotus pulcherrimus was inhibited with ATP at concentrations above 0.2 mM. AMP, ADP, adenosine 3'5'-cyclic phosphate (cyclic AMP) and orthophosphate relieved the enzyme from the ATP-inhibited state. Cyclic AMP was the most effective compound among these agents. Phosphofructokinase activity was measured in the presence of these positive and negative effectors. Concentrations of the effectors were chosen to match the intracellular concentrations of sea urchin eggs at selected post-fertilization periods. The results suggested that changes in phosphofructokinase activity after fertilization were due to changes in the concentrations of these effectors, especially cyclic AMP.

[学科分类] 分子生物学，细胞生物学和基因