Participation of Intercellular Materials in Enhancing Aggregation of Embryonic Quail Liver Cells

[摘要] References(23)The effect of intercellular materials on aggregation of dis-sociated embryonic quail liver cells was examined in rotation-mediated cellcultures in th presence and absence of the medium (dissociation medium) in which cells were dispersed. Transfer of cells to a fresh medium after removal of the dissociation medium resulted in the formation of smaller aggregates than incubation in the dissociation medium, but on addition of the dissociation medium to the fresh medium, the aggregate size was restored. Dissociation medium was not effective in enhancing cell aggregation in the presence of cycloheximide. The cell aggregate size depended on the length of trypsin treatment, with prolonged treatment resulting in smaller aggregates. This decrease in aggregate size with prolonged trypsin treatment was more marked in the presence of cycloheximide.

[学科分类] 分子生物学，细胞生物学和基因