Parasexual Hybridization in Cellular Slime Molds

[摘要] References(23)Cited-By(1)Parasexual hybrids of Dictyostelium discoideum were obtained at a high frequency of 10-2 to 10-3 when stationary phase cells in the primary culture were allowed to multiply secondarily before inoculating onto selective plates. This selection following secondary multiplication demonstrated that hybridization occurred even under standard culture conditions during primary culture. Progenies of the hybrid clones retained genetic markers originating from both parents in a recombined manner. Culturing cells with labelled and non-labelled nuclei together revealed that a certain portion of multinucleated cells appearing in the primary culture resulted from cell fusion. The fused cells gradually increased in number during the stationary phase of primary culture, and the frequency of their appearance was comparable to that of the genetic hybrids that arose in the secondary culture.

[学科分类] 分子生物学，细胞生物学和基因