Mitotic Behaviour of Yeast Spheroplasts in Liquid Culture

[摘要] References(11)Cited-By(3)The mitotic behaviour of spheroplasts prepared from synchronized cells of Saccharomyces cerevisiae IFO 0971 in a liquid medium varied with cell density. When incubated at low cell density, spheroplasts entered the first round of mitosis in partial synchrony and most proceeded to the second round. When incubated at high cell density, they showed significant retardation of mitosis, although they apparently carried out one normal round of DNA synthesis. These and other observations indicate that in spheroplast cells incubated at high cell density, some biochemical reactions necessary for the initiation of mitosis are retarded. An additional observation suggested that spheroplast cells may undergo normal mitosis only in the presence of relatively high concentrations of nutrients in the surrounding medium.

[学科分类] 分子生物学，细胞生物学和基因