Microtubules in Protozoan Cells. IV. Effects of Sulfhydryl-Blocking Reagents on Axopodial Degradation and Reformation in Heliozoa

[摘要] References(25)Cited-By(1)The influence of sulfhydryl-blocking reagents, p-chloromer-curibenzoate (PCMB) and N, N-dimethylamide (diamide), on heliozoan axopodia was examined with particular emphasis on the degradation and reformation mechanism of the axopodia. Diamide had less effect on axopodial degradation at concentrations up to 10-3 M. In contrast, PCMB (10-4 to 10-5 M) rapidly induced almost complete degradation of axopodial microtubules. Lower concentrations of PCMB (10-6 to 10-7 M) caused partial degradation of the axopodia; thus, the degradation effect of PCMB was highly concentration-dependent. 10-3 M diamide inhibited axopodial recovery from partial degradation by 10-5 M PCMB although PCMB did not disturb the recovery process even at the concentration that induced partial degradation of the axo-podia. Some control mechanisms that determine axopodial length and the disand re-assembly of microtubules in vivo are discussed.

[学科分类] 分子生物学，细胞生物学和基因