Fusion of Dissociated Embryonic Cells in the Teleost, Oryzias latipes. IV. Changes in Cell Surface Morphology Related to This Fusion: A Scanning Electron Microscope Study

[摘要] References(10)When isolated embryonic cells of the medaka, Oryzias latipes, were brought into physical contact within 30 sec of dissociation, cell fusion could be induced, but fusion could no longer be induced between cells that had been isolated for more than 90 sec. Observations with a scanning electron microscope revealed that the cell surface was smooth immediately after dissociation. Cells examined about 30 sec after dissociation, however, had a great wealth of surface folds. The cell surface observed about 90 sec after dissociation was again smooth. Fusing cells were highly plicated. At the contact sites of the two fusing cells many plicae were intertwined in complex patterns. The transitory existence of cell surface folds is believed to be a prerequisite for this cell fusion.

[学科分类] 分子生物学，细胞生物学和基因