Conversion of Proliferation and Production of the Colony Stimulating Factor during Serial Passage of Mouse Fibroblasts in Culture

[摘要] References(22)Fibroblast-like cells from a BALB/c mouse embryo were propagated and transferred weekly in culture with a constant inoculation density of 5 × 103 cells/cm2. In this culture schedule a period of decreased proliferation (crisis) continued for 3 to 4 weeks before the initiation of continuous cell proliferation. This provided an experimental system suitable for studying proliferation conversion. The number and the proliferation rate of clonogenic cells increased early in crisis. Production of the colony-stimulating factor for mouse bone marrow cells by fibroblast-like cells was enhanced during the period of decreasing proliferation and crisis, then it decreased when continuous proliferation began. The results indicate that cells with infinite proliferation potential appear early in crisis, although the major population of cells terminates proliferation during crisis and enhances differentiated functions.

[学科分类] 分子生物学，细胞生物学和基因