Population-Dependent Requirements of Vitamin B12 and Metabolically Related Substances of Several Mouse Cell Types in Serum-Free, Albumin-Fortified Medium

[摘要] References(32)Cited-By(2)Mouse FM3A cells propagated well in serum-free medium containing 0.5 % serum albumin and 1 μg of insulin/ml. The vitamin B12 (B12) requirement of the cells depended on the population density. This requirement disappeared when a sufficiently large cell population was present. A combination of 1-100 ng of B12/ml and 4 μg of hypoxanthine/ml resulted in a synergistic increase in cell growth at low cell densities. A similar growth response was obtained when the B12 plus hypoxanthine was replaced by 4 μg of hypoxanthine/ml in combination with 100 ng of thymidine/ml, 1 μg of folic acid/ml or 1 μg of folic acid/ml, even though 1 μg of folic acid/ml already was present in the medium. Experiments on single cell inoculation showed that colony size and the yield of cells grown in B12-supplemented medium were much larger than those for cells grown in B12-free medium. A more critical population-dependent B12 requirement was demonstrated in mouse Ehrlich and L cells and their hybrids. At less than 100 cells there was no propagation in serum-free medium lacking B12, folic acid and thymidine; whereas, a satisfactory growth response was obtained in medium supplemented with these substances.

[学科分类] 分子生物学，细胞生物学和基因