Flow Cytometric Determination of Cell Cycle Parameters of V79 Cells by Continuous Labeling with Bromodeoxyuridine

[摘要] References(29)Cited-By(2)A simple method with which to determine the cell cycle parameters, TG1, TS and TG2M (the durations of the G1, S and G2+M phases) is described. V79 Chinese hamster lung cells were used to evaluate the method. After continuous labeling with bromodeoxyuridine (BrdU), V79 cells were stained with anti BrdU-monoclonal antibody with FITC (fluorescein isothio-cyanate) and with PI (propidium iodide). The individual cells were checked by flow cytometry for green and red fluorescences whose signal intensities cor-responded to the BrdU and cellular DNA contents. The durations of G1, S and G2+M phases of V79 cells were determined by measuring the cell fractions containing the nonlabeled G1, labeled S and nonlabeled G2+M phases. The reliability of this method is discussed.

[学科分类] 分子生物学，细胞生物学和基因