PRETREATMENT OF CELLULOSIC MATERIALS FOR DIGESTION BY RUMINOCOCCUS ALBUS

[摘要] References(19)The effects of chemical pretreatment on cellulose digestion by a cellulolytic anaerobe, Ruminococcus albus, were examined. Pretreatment with H3PO4 was the most effective for digestion of crystalline cellulose by R. albus of the four pretreatment methods investigated. The best condition for pretreatment of Avicel with H3PO4 was as follows; H3PO4 concentration: 85-100 (v/v)%; treatment time: over 5 min; treatment temperature: 0°C at 85 (v/v)% H3PO4 or 0-60°C at 95 (v/v)% H3PO4; cellulose concentration: 1-3 (w/w)%. Treatment efficiencies did not decrease after five repeated uses of H3PO4. For effective digestion of newspaper or rice straw, delignification was essential in addition to swelling with H3PO4. For newspaper and rice straw which were cryomilled, delignified and swollen with H3PO4, specific cellulose digestion rates by R. albus were 0.081 and 0.099 g cellulose/g dry cells/hr, respectively.