Unexpectedly Weak Impacts of Decreased p53 and Retinoblastoma Protein Levels on Mutagenesis by 8-Oxo-7,8-dihydroguanine (8-Hydroxyguanine)

[摘要] References(42)Cited-By(5)The loss of tumor suppressor proteins, p53 and retinoblastoma (Rb), causes genomic instability. 8-Oxo-7,8-dihydroguanine (GO, 8-hydroxyguanine) is a major oxidatively damaged base that induces G:C→T:A transversions in cells. In this study, the effects of p53 and Rb reductions on the mutagenicity of GO in DNA were investigated, using a supF shuttle plasmid propagated in human U2OS and HT1080 cells. The p53 and Rb proteins were individually knocked-down by siRNAs, and then the plasmid DNA containing GO was introduced into the knocked-down cells. The knock-downs of p53 and Rb had quite weak effects on mutagenesis by GO in the shuttle plasmid. These results suggested that p53 and Rb minimally affect the GO-induced mutagenic processes in living cells.

[学科分类] 分子生物学，细胞生物学和基因

[关键词] p53;Rb;8-oxo-7;8-dihydroguanine;8-hydroxyguanine