Oxidative DNA Damage Induced by 364-nm UVA Laser in Yeast Cells

[摘要] References(15)Cited-By(4)The mechanisms of the toxic effects of UVA (320-400 nm) irradiation remain unclear. The actions of monochromatic longer wavelength UVA, in particular, have been difficult to analyze because of a lack of a powerful light source; however, a UVA laser that can be used for biological studies was recently developed. In the current studies, we examined the effects of 364-nm irradiation on yeast cells using a potent UVA laser. We found that, when irradiated under aerobic conditions, yeast cells lacking Ogg1 glycosylase were more sensitive than those with Ogg1. The ability of the 364-nm light to kill the yeast cells was almost eliminated by purging with argon gas. The mutagenic effects of the UVA irradiation did not appear to be enhanced by a lack of Ogg1. These results indicate the killing of yeast cells by 364-nm UVA may be dependent on oxidation and may involve DNA lesions that can be repaired by Ogg1.

[学科分类] 分子生物学，细胞生物学和基因

[关键词] UVA;Ogg1;oxygen;laser;yeast