Furosemide-Sensitive Calcium Rise Induced by GABAA-Receptor Stimulation in Cultures of Embryonic Rat Striatal Neurons

[摘要] References(31)Cited-By(3)Changes in [Ca2+]i induced by γ-aminobutyric acid (GABA)were investigated in primary cultured neurons obtained from fetal rat striatum. GABA and muscimol induced [Ca2+]i rise, and bicuculline blocked the effect of GABA. The [Ca2+]3 elevating effect of GABA was also abolished by removal of extracellular Ca2+ or by application of nicardipine. Furthermore, furosemide, an inhibitor of Na+/K+/ 2Cl- co-transport, reversibly inhibited the GABA-induced [Ca2+]i rise. These results suggest that due to the elevated level of intracellular Cl- maintained by Na+/K+/2Cl- transport activity in these neurons, opening of GABAA-receptor-associated Cl- channels results in Cl- efflux, leading to membrane depolarization and activation of L-type voltage-dependent Ca2+ channels.

[学科分类] 药理学

[关键词] γ-Aminobutyric acid (GABA);Striatal neuron;Intracellular calcium;Chloride transport;Development