Analysis of Dissociated Single Neurons by Simple and Semi-Quantitative RT-PCR (Reverse Transcription and Polymerase Chain Reaction)

[摘要] References(7)Cited-By(4)We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H1-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.

[学科分类] 药理学

[关键词] Histamine H1-receptor mRNA;Single whole-cell PCR;Primary sensory neuron