A METHOD OF ASSAYING ADRENAL CORTICAL HORMONES BASED ON A DECREASE IN THE CIRCULATING LYMPHOCYTES OF HYPOPHYSECTOMIZED RATS (LYMPHOPENIA METHOD)

[摘要] References(10)For the past ten years we have continued our researches in the biological assaying method of adrenal cortical hormones and have been studying the survival growth test, muscle work test, cold test, liver glycogen deposition test, potassiti poisoning test and water intoxication test. Most of these are designed to measure the effectiveness of assayed materials by observing the compensation of a phase of the multifarious functional deficiencies. each having its own feature, but they have unable to constitute the complete indices covering the effectiveness of hormones, and there are liable to creep in many drawbacks in showing the sensitivity or in the assaying technique. It is, therefore, highly desirable to realize the completion of a method of chemical estimation in place of the foregoing biological assaying method. For the present, however, we cannot definitely claim that corticoids or the so called “chemocorticoids” which makes the estimation chemically possible, is the so called “biocorticoids” which is biologically active. What is keenly desirable today above anything else is the accomplishment of a biological assaying method, which is at once accurate, sensitive and rapid, in order to satiate the demand following the remarkable development in the application of adrenal cortical hormones or for the accomplishment of the method of chemical estimation. In order to meet the expectations in this connection we studied extensively, and we turned our attention to the circulating lymphopenia method of 11-oxycorticoid hormone having been announced by Dougherty and White(1) in 1943 and established an assaying method aimed at the decrease of circulating lymphocyte cells of hypophysectomized rats [O-kawara, 1950 (2)]. This method is not one which measures the effectiveness through a phase of compensation of adrenal cortical functional deficiency but is one which makes the extent of decrease of circulating lymphocytes as the indices of effectiveness. This method is yet to be perfected but it is altogether specific, accurate and sensitive, its technique is easily acquirable, the hypophysectomized rats can be used a number of times and that the assay results can be found out in a short time. Having been able to secure cortisone acetate, the authors studied the results which had so far been obtained by adrenal cortical extracts additional improvements having been effected in the meantime.

[学科分类] 药理学