Detection and Characterization of 3H-Clonidine Binding Sites in Coated Vesicles Isolated from Bovine Brain

[摘要] References(14)Cited-By(5)The binding of 3H-clonidine to bovine brain coated vesicles was studied. The binding was reversible and saturable. Saturation studies revealed a one-site interaction: Kd was 8.7 nM and Bmax was 24.5 fmol/mg protein. Noradrenaline, yohimbine and phenoxybenzamine displaced 3H-clonidine binding from the binding sites at concentrations of 10-7-10-4 M. The results indicate that the coated vesicles from the bovine cerebral cortex contain 3H-clonidine binding sites comparable to the low affinity sites of α2-adrenergic receptors in bovine brain membranes.

[学科分类] 药理学