Distribution of α1- and α2-Adrenoceptors in Brush Border and Basolateral Membranes from Rat Kidney Cortical Tubules

[摘要] References(19)Cited-By(3)To examine the localization of α-adrenoceptors within the kidney tubule, binding experiments with 125I-HEAT and 3H-yohimbine were performed using cortical homogenates, basoalteral membranes (BLM) and brush border membranes (BBM). Bmax values for 125I-HEAT or 3H-yohimbine binding were 2.3 or 2.0-fold that of the homogenate in BBM and 8.2- or 10.7-fold in BLM, respectively. Kd values for 125I-HEAT or 3H-yohimbine were not significantly different between BBM and BLM. These results indicate that both types of α-adrenoceptors seem to be localized largely in the BLM.

[学科分类] 药理学