Hypoxia and Reoxygenation-Induced Injury of Renal Epithelial Cells: Effect of Free Radical Scavengers

[摘要] References(15)Cited-By(6)The aim of this study was to characterize injuries of LLC-PK1 and MDCK cells exposed to hypoxia and reoxygenation. Exposure of LLC-PK1 cells to hypoxia reduced the ATP contents and increased the leakage of lactate dehydrogenase (LDH), but MDCK cells had no such injuries. Hypoxia-reoxygenation of LLC-PK1 cells dramatically increased LDH leakage, which was suppressed by free radical scavengers, N, N'' diphenyl p-phenylenediamine, superoxide dismutase and N, N'' dimethylthiourea. These results suggest that use of LLC-PK1 cells has advantages for the investigation of ischemia-reperfusion injury of the kidney as an in vitro model and that generation of oxygen radicals is involved in the cellular injury induced by hypoxia-reoxygenation.

[学科分类] 药理学

[关键词] Oxidative stress;Hypoxia-reoxygenation;Renal epithelial cell