Effect of Recombinant Human Insulin-Like Growth Factor-I on Expression of Glucose Transporters, GLUT 2 and GLUT 4, in Streptozotocin-Diabetic Rat

[摘要] References(15)Cited-By(3)We investigated the effect of recombinant insulin-like growth factor-I (rhIGF-I) on the expression of glucose transporters, GLUT 2 in the liver and GLUT 4 in the heart, in streptozotocin (STZ)-diabetic rats by the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. GLUT 2 and its mRNA in the liver was elevated, whereas GLUT 4 and its mRNA in the heart were decreased in STZ-diabetic rats. A two-week treatment with rhIGF-I mostly restored the expression of GLUT 2 and GLUT 4 to normal rat level. We demonstrated that the effect of IGF-I on the expressions of GLUTs was almost the same as that of insulin.

[学科分类] 药理学

[关键词] Glucose transporter;Reverse transcription-polymerase chain reaction (RT-PCR);Immunohistochemistry