ABSORPTION LOSS OF PEPTIDES TO THE PLASTIC TUBE IN RADIORECEPTOR ASSAY OF CALCITONIN AND β-ENDORPHIN: PROTECTION BY DETERGENTS

[摘要] References(9)Cited-By(5)During development of the calcitonin binding assay using rat brain particulate fraction, it was noticed that 125I-salmon calcitonin-I (125I-SCT) was partly absorbed by the polypropylene tube surface in an irreversible manner. Absorption was partially prevented by cold SCT and complete prevention was achieved by bacitracin, Triton X-100, Brij 36T, and some Zwittergents in such concentration ranges which appeared not to disrupt the 125I-SCT binding ability of brain tissue. The brain fraction itself exhibited a similar preventive effect. The anti-absorbing effect of Brij 36T was also observed in an opiate receptor binding assay for β-endorphin. These results led us to recommend that the binding assay for these peptides should be done only in the presence of an appropriate antiabsorbant.

[学科分类] 药理学