Alteration of Liver Glutathione S-Transferase and Protease Activities by Cobalt Chloride Treatment of Rats

[摘要] References(26)Cited-By(2)Effects of cobalt chloride on liver glutathione S-transferase and protease activities were studied. When cobalt chloride (60 mg/kg) was given to rats, liver microsomal glutathione S-transferase and protease activities were significantly increased 24 hr after the injection, whereas glutathione peroxidase activity in microsomes was decreased. The increase in glutathione S-transferase activity caused by cobalt chloride treatment was not reversed by dithiothreitol, and the activation of the transferase by N-ethylmaleimide was similar to that of the control, indicating that the increase in the transferase activity by cobalt chloride is not due to a modification of the sulfhydryl group of the enzyme. Immunochemical analysis of the liver microsomes did not detect any proteolytic product of microsomal glutathione S-transferase. In puromycinor actinomycin D-treated rats, an increase in the transferase activity caused by cobalt chloride treatment was depressed. Thus it was suggested that liver microsomal glutathione S-transferase is induced by cobalt chloride treatment, but not activated by limited proteolysis via microsomal protease.

[学科分类] 药理学

[关键词] Cobalt chloride;Glutathione S-transferase;Liver microsome;Enzyme induction;Protease