Inositol 1, 4, 5-Trisphosphate-Induced Ca2+-Transient and Outward K+ Current in Single Smooth Muscle Cells of Guinea Pig Small Intestine

[摘要] References(20)The effects of inositol 1, 4, 5-trisphosphate (InsP3) released from caged InsP3 by flash photolysis on free intracellular Ca2+ concentration, [Ca2+]i, and outward K+ current were simultaneously examined in a single smooth muscle cell of guinea pig small intestine using a patch pipette solution containing Indo-1 (0.1 mM), caged InsP3 (50 μM) and KCl (130 mM). At a holding potential of −50 mV, a depolarizing pulse to + 10 mV for 200 msec caused a transient Ca2+ current and an increase in [Ca2+]i. The amplitude of the Ca2+-transient was positively correlated with the peak Ca2+ current and negatively correlated with resting [Ca2+]i. InsP3 produced increases in [Ca2+]i and outward K+ current in most of the cells at −50 and −30 mV. The outward K+ current response reached a peak sooner and decayed more quickly than the Indo-1 signal. Both responses to InsP3 were resistant to the removal of extracellular Ca2+. The Ca2+-transient and outward K+ current responses to InsP3 at −30 mV were larger than those at −50 mV. The InsP3-induced Ca2+-transient was increased by increasing resting [Ca2+]i at −30 mV but not at −50 mV. These results suggest that InsP3-induced Ca2+ release from stores is potentiated by slight increases in [Ca2+]i via membrane depolarization.

[学科分类] 药理学

[关键词] Caged inositol 1;4;5-trisphosphate;Ca2+-transient;Outward K+ current;Indo-1;Whole-cell patch clamp