The Mode of Inhibitory Action of α-Mangostin, a Novel Inhibitor, on the Sarcoplasmic Reticulum Ca2+-Pumping ATPase from Rabbit Skeletal Muscle

[摘要] References(12)Cited-By(9)α-Mangostin, the principal ingredient of the fruit hull of Garcinia mangostana, caused a concentration-dependent decrease in the activities of both Ca2+-ATPase and Ca2+-transport of the sarcoplasmic reticulum from rabbit skeletal muscle with an IC50 value of 5 μM. Neither Ca2+ release nor other enzyme activities were affected by α-mangostin. Kinetic analysis of the inhibitory effects of α-mangostin on Ca2+-ATPase suggests that the inhibition of the ATPase is a noncompetitive-type with respect to ATP or Ca2+. α-Mangostin may become a useful pharmacological tool for clarifying the physiological functions of Ca2+-pumping ATPase and sarcoplasmic reticulum.

[学科分类] 药理学

[关键词] α-Mangostin;Ca2+-pumping ATPase;Sarcoplasmic reticulum