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Affinity Purification of Macrophage Migration Inhibitory Factor/Glycosylation Inhibiting Factor (MIF/GIF) from Bovine Brain by Using a Peptide Ligand Derived from a Novel Serpin
[摘要] References(15)Cited-By(4)We purified macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using an affinity column with the C-terminal region peptide of a novel serpin as a ligand. The affinity purified preparation showing a single band on SDS-PAGE contained four peptides on RP-HPLC, which were converged into two peptides time-dependently. Sequence analysis and Western blotting revealed that one was identical to bovine MIF/GIF and the other was an N-terminally modified form of MIF/GIF. These results indicated that there exist at least two forms of MIF/GIF in the bovine brain and that they have an affinity for the C-terminal portion of the serpin.
[发布日期]  [发布机构] 
[效力级别]  [学科分类] 药理学
[关键词] Macrophage migration inhibition factor/glycosylation inhibiting factor (MIF/GIF);Brain;Serpin [时效性] 
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